Statistical Optimization Of Process Parameters For  Polygalacturonase Production By Bacillus Subtilis Kp10b

Kavisha K Patidar; Swati A Peshwe

doi:10.48165/

Authors

Kavisha K Patidar Department of Microbiology, Government Institute of Science, Aurangabad - 431 001, Maharashtra (India)
Swati A Peshwe Department of Microbiology, Government Institute of Science, Aurangabad - 431 001, Maharashtra (India)

DOI:

https://doi.org/10.48165/

Keywords:

Bacillus subtilis, central composite design, Plackett-Burman design, polygalacturonase, statistical optimization

Abstract

In present study the statistical optimization of process parameters for cost effective production of polygalacturonase (PGase) by Bacillus subtilis was examined by using Plackett-Burman design (PBD) and central composite design (CCD). Of the 9 factors, temperature, CaCO3 and KH2PO4 were found significant by PBD and were further optimized by CCD. After optimization, maximal PGase activity (272.87 Um L-1) was achieved at 40°C, 3.5 mM concentration of KH2PO4 and 6.5 mM concentration of CaCO3. The results showed 2.95-fold increase in PGase activity of B. subtilis under optimized conditions. Thus, it may be concluded that optimized medium is cost-effective and suitable for maximum production and may be used for large-scale PGase production by B. subtilis KP10B. The crude enzyme extracts of B. subtilis can be used in industry for fruit juice clarification, waste water treatment and coffee fermentation.

Downloads

Download data is not yet available.

References

Ahmed, T., Rana, M.R., Zzaman, W., Ara, R. and Aziz, M.G. 2021. Optimization of substrate composition for pectinase production from Satkara (Citrus macroptera) peel using Aspergillusniger - ATCC1640 in solid-state fermentation. Heliyon, 7(10): e08133. [http://doi:10.1016/j.heliyon.2021.e08133].

Bibi, N., Ali, S. and Tabassum, R. 2016. Statistical optimization of pectinase biosynthesis from orange peel by Bacilluslicheniformis using submerged fermentation. Waste and Biomass Valorization, 7(3): 467-481.

El-Enshasy, H.A., Elsayed, E.A., Suhaimi, N., Malek, R.A. and Esawy, M. 2018. Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system. BMC Biotechnology, 18(1): 1-13.

Gupta, R., Kohli, P. and Sharma, N. 2016. Statistical optimization of production conditions of alkaline pectin lyase from Bacillus cereus using response surface methodology. Journal of Food and Industrial Microbiology, 2: 113. [https://doi:10.4172/2572-4134.100011310].

Kavuthodi, B. and Sebastian, D. 2018. Biotechnological valorization of pineapple stem for pectinase production by Bacillus subtilis BKDS1: Media formulation and statistical optimization for submerged fermentation. Biocatalysis and Agricultural Biotechnology, 16: 715-722.

Kuvvet, C., Uzuner, S. and Cekmecelioglu, D. 2019. Improvement of pectinase production by co culture of Bacillus spp. using apple pomace as a carbon source. Waste and Biomass Valorization, 10: 1241-1249.

Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3): 426-428.

Mohandas, A., Raveendran, S., Parameswaran, B., Abraham, A., Athira, R.S., Mathew, A.K. and Pandey, A. 2018. Production of pectinase from Bacillus sonorensis MPTD1. Food Technology and Biotechnology, 56(1): [https://doi:10.17113/ftb.56.01.18.5477].

Namasivayam, E., Ravindar, J.D., Mariappan, K., Akhil, J., Mukesh, K. and Jayaraj, R. 2011. Production of extracellular pectinase by Bacillus cereus isolated from market solid waste. Journal of Bioanalysis Biomedicine, 3(3): 70-75.

Narayanan, M., Rangarajan, V., Arunkumar, M. and Shrinivas, B.A.N. 2014. Statistical designing of enriched pectin extract medium for the enhanced production of pectinase by Aspergillus niger. International Journal of Pharmacy and Pharmaceutical Sciences, 6: 666-672.

Nawawi, M.H., Ismail, K.I., Sa’ad, N., Mohamad, R., Tahir, P.M., Asa’ari, A.Z. and Saad, W.Z. 2022. Optimisation of xylanase–pectinase cocktail production with Bacillus amyloliquefaciens ADI2 using a low-cost substrate via statistical strategy. Fermentation, 8: 119. [https://doi.org/10.3390/fermentation8030119].

Oumer, O.J. and Abate, D. 2018. Comparative studies of pectinase production by Bacillus subtilis strain BTK27 in submerged and solid-state fermentations. BioMed Research International, 2018: [https://doi.org/10.1155/2018/1514795].

Patidar, M.K., Nighojkar, A., Nighojkar, S. and Kumar, A. 2017. Purification and characterization of polygalacturonase produced by Aspergillus niger AN07 in solid state fermentation. Canadian Journal of Biotechnology, 1(1): 11-18.

Rahman, R.N.Z., Geok, L.P., Basri, M. and Salleh, A.B. 2005. Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource Technology, 96(4): 429-436.

Sharma, D.C. and Satyanarayana, T. 2012. Biotechnological potential of agro residues for economical production of thermoalkali-stable pectinase by Bacillus pumilus DCSR1 by solid state fermentation and its efficacy in the treatment of ramie fibres. Enzyme Research, 2012: [http://doi.org/10.1155/2012/281384].

Sharma, D.C. and Satyanarayana, T. 2006. A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus DCSR1 in submerged fermentation by using statistical methods. Bioresource Technology, 97(5): 727-733.

Shrestha, S., Rahman, M.S. and Qin, W. 2021. New insights in pectinase production development and industrial applications. Applied Microbiology and Biotechnology, 105 (24): 9069–9087. Spanos, N. and Koutsoukos, P.G. 1998. Kinetics of precipitation of calcium carbonate in alkaline pH at constant supersaturation. Spontaneous and seeded growth. The Journal of Physical Chemistry B, 102(34): 6679-6684.

Tepe, O. and Dursun, A.Y. 2021. Optimization of endo-pectinase and pectin lyase production from wheat bran by Bacillus pumilus using response surface methodology. Gazi University Journal of Science, 34(2): 335-353.

Uzuner, S. and Cekmecelioglu, D. 2015. Enhanced pectinase production by optimizing fermentation conditions of Bacillus subtilis growing on hazelnut shell hydrolyzate. Journal of Molecular Catalysis B: Enzymatic, 113: 62-67.

Wong, L.Y., Saad, W.Z., Mohamad, R. and Tahir, P. Md. 2017. Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf. Industrial Crops and Products, 97: 175-183.

Yadav, K.K., Garg, N., Kumar, D., Kumar, S., Singh, A. and Muthukumar, M. 2015. Application of response surface methodology for optimization of polygalacturonase production by Aspergillus niger. Journal of Environmental Biology, 36(1): 255-259.