In Vitro Clonal Propagation Of Pomegranate (Punica Granatum L.)  Cv. Bhagwa

R Kiruthika Devi; C Kavitha; K Soorianathasundaram; K Hemaprabha

doi:10.48165/

Authors

R Kiruthika Devi Department of Fruit Science, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu (India)
C Kavitha Department of Fruit Science, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu (India)
K Soorianathasundaram Department of Fruit Science, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu (India)
K Hemaprabha Department of Fruit Science, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu (India)

DOI:

https://doi.org/10.48165/

Keywords:

Micropropagation, nodal segment, phenolic exudation, pomegranate, rooting, shoot tip

Abstract

An attempt was made to standardize in vitro clonal propagation of pomegranate cv. Bhagwa, using nodal segment and shoot tip explants at e, India. Direct organogenesis of pomegranate was tried in MS medium with growth regulator combinations viz., benzyl amino purine (BAP), α-naphthalene acetic acid (NAA) and adenine sulphate for in vitro shoot regeneration and indole butyric acid (IBA) for in vitro rooting. Among the explants evaluated, nodal segments responded better for culture establishment. Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 min gave highest survival rate (81.7%) with minimum microbial contamination (13.3%). Phenolic exudation was reduced by treating explants in antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 min and then cultured on basal MS medium with polyvinyl pyrrolidone (100 mg L-1) resulted in maximum survival (98.3%). The earliest shoot evocation response (4.4 days), higher shoot proliferation (91.7%) and maximum shoot number (4.6) were observed on basal MS medium with adenine sulphate (40 mg L1). Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm). Rooted plantlets transferred to hardening media with pot mixture and vermicompost (1:1) exhibited an ex-vitro survival of 81.7%.

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References

Ahmad, L.N., Faisal, M., Anis, M. and Aref, I.M. 2013. In vitro callus induction and plant regeneration fromleaf explants ofRuta graveolens. South African Journal Botany, 76: 597-600. Bachake, S.S., Jadhav, V.B., Deshpande, P.P., Tele, A.A., Banda, M.A., Adki, V.S., Gopika, M.K.,

Karanjule, P.G., Birajdar, S.B., Karwa, N.N., Mundhewadikar, D.M. and Singh, N.V. 2019. Standardization of in vitro propagation protocol for pomegranate cv. Super Bhagwa. Journal of Pharmacognosy and Phytochemistry, 8(3): 2548-2553.

Bharose, A.A., Khandagale D.J. and Damse, D.N. 2014. Regeneration media standardization of pomegranate (Punica granatum L.) cv. Bhagwa from shoot tip, cotyledonary node, hypocotyls and leaves. Vegetos, 27(2): 338-342.

Bonyanpour, A. and Khosh-Khui, M. 2013. Callus induction and plant regeneration in Punica granatum L. ‘Nana’ from leaf explants. Journal of Central European Agriculture, 14: 928-936. Damino, C., Padro, M.D.A. and Frattarelli, A. 2008. Propagation and establishment in vitro of myrtle (Myrtus communis L.), pomegranate (Punica granatum L.) and mulberry (Morus alba L.). Propagation of Ornamental Plants, 8: 3-8.

Deepika, R. and Kanwar, K. 2010. In vitro regeneration of Punica granatum L. plants from different juvenile explants. Journal of Fruit and Ornamental Plant Research, 18: 5-22. Gaiker, P.S. and Yadav, J. 2022. Existing position of pomegranate production among fruits in India. Journal of Emerging Technologies and Innovative Research, 9(1): 314-317.

In vitro clonal propagation of pomegranate cv. Bhagwa 217

Gil, M.I., Tomas-Barberan, F.A., Hess-Pierce, B., Holcroft, D.M. and Kader, A.A. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agriculture and Food Chemistry, 48: 4581-4589.

Hedge, G.N. 2021. Role of non-government organization in horticultural development in India. Indian Horticulture, 66(5): 59-62.

Heloir, M.C., Fournioux, J.C. Oziol, L. and Bessis, R. 1997. An improved procedure for the propagation in vitro of grapevine (Vitis vinifera cv. Pinot noir) using axillary-bud microcuttings. Plant Cell Tissue and Organ Culture, 49: 223-225.

Johanningsmeier, S.D. and Harris, G.K. 2011. Pomegranate as a functional food and nutraceutical source. Annual Reviews of Food Science Technology, 2: 181-201.

Kalabandi, B.M., Waskar, D.P., Khandare, V.S. and Gorad, D.S. 2014. Micropropagation studies on pomegranate var. Bhagwa. Indian Journal of Horticulture, 71(4): 564-566.

Kantharajah, A., Dewitz, I. and Jabbari, S. 1998. The effect of media, plant growth regulators and source of explants on in vitro culture of pomegranate (Punica granatum L.). Erwerbs-obtbau, 40: 54-58.

Kanwar, K., Joseph, J. and Deepika, R. 2010. Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell Tissue and Organ Culture, 100: 199-207.

Krishna, H., Sairam, R.K., Singh, S.K., Patel, V.B., Sharma, R.R., Grover, M. and Sachdev, A. 2008. Mango explant browning: Effect of ontogenic age, mycorrhization and pre-treatments. Scientia Horticulturae, 118(2): 132-138.

Murkute, A.A., Patil, S., Patil, B.N. and Kumari, M. 2002. Micropropagation in pomegranate, callus induction and differentiation. South Indian Horticulture, 50(1-3): 49-55.

Naik S.K., Pattnaik, S. and Chand, P.K. 1999. In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree. Scientia Horticulturae, 79: 175-183.

Naik, S.K. and Chand, P.K. 2011. Tissue culture-mediated biotechnological intervention in pomegranate: A review. Plant Cell Reports, 30: 707-721.

Naik, S.K., Pattnaik, S. and Chand, P.K. 2000. High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Scientia Horticulturae, 85: 261-270.

Naik, S.K. and Chand, P.K. 2003. Silver nitrate and aminoethoxy vinyl glycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). Journal of Plant Physiology, 160(4): 423-430.

Omar, R., Abdullah, M.A., Hasan, M.A. and Marziah, M. 2004. Development of growth medium for Centella asiatica cell culture via. response surface methodology. American Journal Applied Science, 1(3): 215-219.

Panaia, M. 1998. Rescuing Symonanthes bancroftii (Solanaceae), A Western Australian Native Species from Extinction Through In Vitro Micropropagation. Honours Thesis, University of Western Australia, Perth, Australia.

Parth, D., Ghanshyam, P., Bhumika, D., Shikha, D., Fenil, P. and Subhash, N. 2018. Development of an efficient micropropagation protocol through axillary shoot proliferation for pomegranate variety Bhagwa. Annals of Agrarian Science, 16: 444-445.

Patil, V.M., Dhande, G.A., Thigale, D.M. and Rajput, J.C. 2011. Micropropagation of pomegranate (Punica granatum L.) ‘Bhagava’ cultivar from nodal explant. African Journal of Biotechnology, 10(79): 18130-18136.

Preece, J.E. and Compton, M.E. 1991. Problems with explants exudation in micropropagation. Biotechnology in Agriculture and Forestry, 17: 168-189.

Rachana, M.D., Ashok, K.P., Vibha, J. B., Smita, S. and Shekhawat, N.S. 2019. Cloning of mature pomegranate (Punica granatum) cv. Jalore seedless via in vitro shoot production and ex vitro rooting. International Journal of Plant Research, 2(32): 181-189.

R. Kiruthika Devi et al.

Satheesh, M. and Sridharan, T.B. 2014. Standardization of Punica granatum explants and callus induction through micropropagation - Indirect organogenesis. International Journal of Research in Engineering Technology, 3(1): 19-27.

Shao, J.Z., Chen, C.L. and Deng, X.X. 2003. In vitro induction of tetraploid in pomegranate (Punica granatum). Plant Cell Tissue and Organ Culture, 75: 241-246.

Sharon, M. and Sinha, S. 2000. Plant regeneration from cotyledonary node of Punica granatum L. Indian Journal of Plant Physiology, 5: 344-348.

Singh, V.P.K., Chauhan, P., Kumari, P. and Seema K. 2011. Identification and prevention of bacterial contamination on explant used in plant tissue culture labs. Alcohol, 3(4): 160-163. Singh, J., Gupta, S. and Khoshe, P. 2014. In vitro regeneration of pomegranate (Punica granatum L.) from nodal explant. International Journal of Advance Pharmacy Biology Chemical, 3: 734- 736.

Singh, P. and Patel, R.M. 2014. Factors influencing in vitro growth and shoot multiplication of pomegranate. Journal of Life Science., 9(3): 1031-1035.

Singh, P., Patel, R.M. and Kadam, S. 2013. In vitro mass multiplication of pomegranate from cotyledonary nodal explants cv. Ganesh. African Journal of Biotechnology, 12(20): 2863-2868. Singh, S.K., Khawale, R.N. and Singh, S.P. 2002. Effect of season, type of explants and pre treatments to minimize polyphenolics exudation on in vitro culture establishment in grape. Indian Journal Horticulture, 59: 233-238.

Singh, S.K., Singh, A., Singh, N.V. and Ramajayam, D. 2010. Pomegranate tissue culture and biotechnology. Fruits, Vegetables, Cereal Science and Biotechnology, 4: 35-44. Soukhak, F., Khaligh, A. and Ghaemmaghami, S.A. 2011. Study of direct adventious shoot regeneration in pomegranate (Punica granatum cv. Malas Saveh) through cotyledonary explants. International Journal of Agricultural Science and Research, 2: 19-26. Suhasini, S.C., Patil, S.N., Prabhuling, G., Venkateshalu and Jagadeesh, S.L. 2017. A suitable explant for culture establishment in pomegranate (Punica granatum L.) Advances in Bioresearch, 8(5): 33-36.

Sukhamate, P. and Panse, V. 1954. Statistical Methods for Agricultural Workers. Indian Council of Agricultural Research, New Delhi, India.

Susmita, S., Taramla, R. and Shiv, K. 2016. A review on the scope for increasing in vitro production of pomegranate (Punica granatum L.) cultivars and its application in the human health sector with emphasis on the Indian industry. Plant Cell Biotechnology and Molecular Biology, 16(1&2): 60-73.

Tewari, P. and Avinashilingam, V. 2020. Supply chain of pomegranate and its value addition: Impact of improved variety Bhagwa. Advances in Agriculture Horticulture and Entomology, 111: 1-6.

Tiwari, S.K., Tiwari, K.P. and Siril, E.A. 2012. An improved micropropagation protocol for teak. Plant Cell Tissue Organ Culture, 71: 1-6.

Usharani, T.R., Meenakshi, S., Vijayalaksmi, Sunisha, C. and Mohandas, S. 2014. In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv. Bhagwa. Advance in Applied Research, 6(1): 66-71.

Van Staden, J. and Drewes, F. 1992. The stability and metabolism of benzyl adenine glucosides in soyabean callus. Journal of Plant Physiology, 140: 92-95.