Antagonistic Role Of Fungal Biocontrol Agents For Sustain Able Management Of Rice Blight Caused By Curvularia Lunata

Monalisha Sarkar; Zerald Tiru; Ayon Pal; Parimal Mandal

doi:10.48165/

Authors

Monalisha Sarkar Mycology and Plant Pathology Laboratory, Department of Botany, Raiganj University, Raiganj, Uttar Dinajpur – 733 134, West Bengal (India)
Zerald Tiru Mycology and Plant Pathology Laboratory,Department of Botany, Raiganj University, Raiganj, Uttar Dinajpur – 733 134, West Bengal (India)
Ayon Pal Microbiology and Computational Biology Laboratory, Department of Botany, Raiganj University, Raiganj, Uttar Dinajpur – 733 134, West Bengal (India)
Parimal Mandal Mycology and Plant Pathology Laboratory,Department of Botany, Raiganj University, Raiganj, Uttar Dinajpur – 733 134, West Bengal (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antagonism, defense enzymes, hydrogen cyanide, plant growth promoting fungi, rhizosphere

Abstract

Plant growth promoting fungi (PGPF) have received much attention in recent past due to their plant growth promoting ability and antagonistic activity against disease causing pathogens. They can suppress the pathogen directly by antagonistic mechanism or indirectly by inducing systemic resistance. The present study was aimed to assess the biocontrol efficacy of rhizospheric fungi, isolated from rice fields, against rice blight pathogen Curvularia lunata. Dual culture method was used to assess the antagonistic potential of isolates against the pathogen. Out of the 20 isolates, 14 isolates exerted positive effects against C. lunata in dual culture. SEM studies of interacting zone between the pathogen and isolates in dual culture revealed the mycoparasitic property of Trichoderma aureoviride TaN16 and Aspergillus niger AnD6 against the pathogenic fungus. These two isolates were found associated with HCN production. Three fungal isolates viz., T. aureoviride TaN16, T. yunnanense TaN17 and Talaromyces purpureogenus TpG11 triggered the induction of defense enzymes like phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase. The study revealed that the rhizospheric fungal isolates having biocontrol potential can serve as alternative to chemical fungicides under integrated disease management.

Downloads

Download data is not yet available.

References

Abd El-Rahman, A.F., Shaheen, H.A., Abd El-Aziz, R.M. and Ibrahim, D.S.S. 2019. Influence of hydrogen cyanide-producing rhizobacteria in controlling the crown gall and root-knot nematode, Meloidogyne incognita. Egyptian Journal of Biological Pest Control, 29: 41-47.

Aktar, M.W., Sengupta, D. and Chowdhury, A. 2009. Impact of pesticides use in agriculture: Their benefits and hazards. Interdisciplinary Toxicology, 2: 1-12.

Benítez, T., Rincón, A.M., Limón, M.C. and Codón, A.C. 2004. Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7: 249-260.

Bhandari, S., Pandey, K.R., Joshi, Y.R. and Lamichhane, S.K. 2021. An overview of multifaceted role of Trichoderma spp. for sustainable agriculture. Archives of Agriculture and Environmental Science, 6: 72-79.

Campos, R.P.C. and Jacob, J.K.S. 2021. Biocontrol potential of endophytic Aspergillus spp. against Fusarium verticillioides. Biotropia - The Southeast Asian Journal of Tropical Biology, 28: 141-148.

Choi, H.W. and Ahsan, S.M. 2022. Biocontrol activity of Aspergillus terreus ANU-301 against two distinct plant diseases, tomato Fusarium wilt and potato soft rot. The Plant Pathology Journal 38: 33-45.

Coninck, E., Scauflaire, J., Gollier, M., Liénard, C., Foucart, G., Manssens, G., Munaut, F. and Legrève, A. 2020. Trichoderma atroviride as a promising biocontrol agent in seed coating for reducing Fusarium damping-off on maize. Journal of Applied Microbiology, 129: 637-651.

Coşkuntuna, A. and Özer, N. 2008. Biological control of onion basal rot disease using Trichoderma harzianum and induction of antifungal compounds in onion set following seed treatment. Crop Protection, 27: 330-336.

El-Debaiky, S.A.E.K. and El-Badry, S.M.A. 2021. Lytic Enzymes of Aspergillus piperis as a tool for attacking some phytopathogenic fungi in vitro with special reference to its cytotoxicity. Journal of Pure and Applied Microbiology, 15: 1947-1957.

Elsharkawy, M.M., Nakatani, M., Nishimura, M., Arakawa, T., Shimizu, M. and Hyakumachi, M. 2015. Suppression of rice blast, cabbage black leaf spot, and tomato bacterial wilt diseases by Meyerozyma guilliermondii TA-2 and the nature of protection. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science, 65: 629-636.

Elsharkawy, M.M., Sakran, R.M., Ahmad, A.A., Behiry, S.I., Abdelkhalek, A., Hassan, M.M. and Khedr, A.A. 2022. Induction of systemic resistance against sheath blight in rice by different Pseudomonas isolates. Life, 12(3): 349; [doi: 10.3390/life12030349].

Fokkema, N.J. 1976. Antagonism between fungal saprophytes and pathogens on aerial plant surfaces. pp. 487-506. In: Microbiology of Aerial Plant Surfaces. (eds. C.H. Dickinson and T.F. Preece). Academic Press, London, UK.

Gayoso, C., Pomar, F., Novo-Uzal, E., Merino, F. and de Ilárduya, O.M. 2010. The Ve-mediated resistance response of the tomato to Verticillium dahliae involves H2O2, peroxidase and lignins and drives PAL gene expression. BMC Plant Biology 10: 1-19.

Habibah, J., Lee, P.T., Khairiah, J., Ahmad, M.R., Fouzi, B.A. and Ismail, B.S. 2011. Speciation of heavy metals in paddy soil from selected areas in Kedah and Penang, Malaysia. African Journal of Biotechnology, 10(62): 13505-13513.

Hata, E.M., Yusof, M.T. and Zulperi, D. 2021. Induction of systemic resistance against bacterial leaf streak disease and growth promotion in rice plant by Streptomyces shenzhenesis TKSC3 and Streptomyces sp. SS8. Plant Pathology Journal, 37: 173-181.

Hossain, M.M., Sultana, F., Kubota, M., Koyama, H., and Hyakumachi, M. 2007. The plant growth-promoting fungus Penicillium simplicissimum GP17-2 induces resistance in Arabidopsis thaliana by activation of multiple defense signals. Plant Cell Physiology, 48, 1724-1736.

Fungal bioagents for management of rice blight 201

Jogaiah, S., Abdelrahman, M., Tran, L.S. and Shin-ichi, I. 2013. Characterization of rhizosphere fungi that mediate resistance in tomato against bacterial wilt disease. Journal of Experimental Botany, 64: 3829-3842.

Johnson, L.F. and Curl, E.A. 1972. Methods for research on the ecology of soil-borne plant pathogens. Methods for research on the ecology of soil-borne plant pathogens. Tennessee University, Knoxville, USA

Kagale, S., Marimuthu, T., Kagale, J., Thayumanavan, B. and Samiyappan, R. 2011. Induction of systemic resistance in rice by leaf extracts of Zizyphus jujuba and Ipomoea carnea against Rhizoctonia solani. Plant Signal Behaviour, 6: 919-923.

Kalyabina, V.P., Esimbekova, E.N., Kopylova, K.V. and Kratasyuk, V.A. 2021. Pesticides: Formulants, distribution pathways and effects on human health – A review. Toxicology Reports 8: 1179-1192.

Konappa, N., Krishnamurthy, S., Siddaiah, C., Ramachandrappa, N. and Chowdappa, S. 2018. Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solaniacearum. Egyptian Journal of Biological Pest Control, 28: 1-11.

Kumar, N., and Khurana, S.M. 2021. Trichoderma-plant-pathogen interactions for benefit of agriculture and environment. pp. 41-63. In: Biocontrol Agents and Secondary Metabolites. Woodhead Publ. [https://doi.org/10.1016/B978-0-12-822919-4.00003-X].

Lalève, A., Gamet, S., Walker, A.S., Debieu, D., Toquin, V. and Fillinger, S. 2014. Site-directed mutagenesis of the P225, N230 and H272 residues of succinate dehydrogenase subunit B from Botrytis cinerea highlights different roles in enzyme activity and inhibitor binding. Environmental Microbiology, 16: 2253-2266.

Lavanya, S.N., Niranjan-Raj, S., Jadimurthy, R., Sudarsan, S., Srivastava, R., Tarasatyavati, C., Rajashekara, H., Gupta, V.K. and Nayaka, S.C. 2022. Immunity elicitors for induced resistance against the downy mildew pathogen in pearl millet. Scientific Reports, 12(1): 1-17.

Li, M.H. 2003. Peroxidase and superoxide dismutase activities in fig leaves in response to ambient air pollution in a subtropical city. Archives of Environmental Contamination and Toxicology, 45, 168-176.

Limtong, S., Into, P. and Attarat, P. 2020. Biocontrol of rice seedling rot disease caused by Curvularia lunata and Helminthosporium oryzae by epiphytic yeasts from plant leaves. Microorganisms, 8(5): 647 [DOI:10.3390/microorganisms8050647].

Lorck, H. 1948. Production of hydrocyanic acid by bacteria. Physiologia Plantarum, 1: 142-146. Mahadevan, S. 1996. Average reward reinforcement learning: Foundations, algorithms and empirical results. Machine learning, 22, 159-195.

Mandal, P. and Sadhukhan, S. 2019. Carbon dioxide the green house gas and mushroom fruiting. Review of Research, 8(4): 71-78.

Mendoza, J.L.H., Pérez, M.I.S., Prieto, J.M.G., Velásquez, J.D.Q., Olivares, J.G.G. and Langarica, H.R.G. 2015. Antibiosis of Trichoderma spp strains native to northeastern Mexico against the pathogenic fungus Macrophomina phaseolina. Brazilian Journal of Microbiology, 46, 1093- 1101.

Moreno-Ruiz, D., Lichius, A., Turrà, D., Di Pietro, A. and Zeilinger, S. 2020. Chemotropism assays for plant symbiosis and mycoparasitism related compound screening in Trichoderma atroviride. Frontiers in Microbiology, 11: 3006.[ https://doi.org/10.3389/fmicb.2020.601251].

Muslim, A., Hyakumachi, M., Kageyama, K. and Suwandi, S. 2019. Induction of systemic resistance in cucumber by hypovirulent binucleate Rhizoctonia against anthracnose caused by Colletotrichum orbiculare. Tropical Life Science Research, 30: 109-122.

Pieterse, C., Zamioudis, C., Berendsen, R., Weller, D., van Wees, S. and Bakker, P. 2014. Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology, 52: 347-375. Potshangbam, M., Devi, S.I., Sahoo, D. and Strobel, G.A. 2017. Functional characterization of endophytic fungal community associated with Oryza sativa L. and Zea mays L. Frontiers in Microbiology, 8: 325. [ https://doi.org/10.3389/fmicb.2017.00325].

Monalisha Sarkar et al.

Pratap Singh, S., Keswani, C., Singh, P., Sansinenea, E. and Xuan Hoat, T. 2021. Trichoderma spp. mediated induction of systemic defense response in brinjal against Sclerotinia sclerotiorum. Current Research in Microbial Sciences, 2: 100051. [https://doi.org/10.1016/j.crmicr.2021.100051].

Ramírez-Valdespino, C.A. and Orrantia-Borunda, E. 2021. Trichoderma and nanotechnology in sustainable agriculture: A review. Frontiers in Fungal Biology, 2: 2021 [https://doi.org/10.3389/ffunb.2021.764675].

Ray, H., Douches, D. and Hammerschmidt, R. 1998. Transformation of potato with cucumber peroxidase: Expression and disease response. Physiological and Molecular Plant Pathology, 53: 93-103.

Reithner, B., Ibarra-Laclette, E., Mach Robert, L. and Herrera-Estrella, A. 2011. Identification of mycoparasitism-related genes in Trichoderma atroviride. Applied and Environmental Microbiology, 77: 4361-4370.

Sadasivan, S. and Manickam, A. 1996. Pigments. pp. 190-191. In: Biochemical Methods (2nd edn.). New Age International, New Delhi, India.

Sohn, S.I., Pandian, S., Oh, Y.J., Kang, H.J., Cho, W.S. and Cho, Y.S. 2021. Metabolic engineering of isoflavones: An updated overview. Frontiers in Plant Science, 12: 670102 [doi: 10.3389/fpls.2021.670103].

Sudisha, J., Niranjana, S., Umesha, S., Prakash, H. and Shetty, H.S. 2006. Transmission of seed borne infection of muskmelon by Didymella bryoniae and effect of seed treatments on disease incidence and fruit yield. Biological Control, 37: 196-205.

Tiru, Z., Sarkar, M., Pal, A, Chakraborty, A.P. and Mandal, P. 2021. Three dimensional plant growth promoting activity of Trichoderma asperellum in maize (Zea mays L.) against Fusarium moniliforme. Archives of Phytopathology and Plant Protection, 54: 764-781.

Zhang, Y., Chen, F.S., Wu, X.Q., Luan, F.G., Zhang, L.P., Fang, X.M., Wan, S.Z., Hu, X.F. and Ye, J.R. 2018. Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments. PloS one, 13(7): e0199625. [https://doi.org/10.1371/journal.pone.0199625].

Zin, N.A. and Badaluddin, N.A. 2020. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences 65: 168-178.