Synthesis Of Curcumin Loaded Chitosan Nanoparticles, Their Characterization And Anticancer Activity
DOI:
https://doi.org/10.48165/Keywords:
Breast cancer, ionic gelation method, FTIR, SEM, XRD, MCF-7 cellsAbstract
This study was designed to encapsulate curcumin in chitosan nanoparticles synthesized by ionic gelation method. In dissolution studies, curcumin was found insoluble in water, slightly soluble in HCl, H2SO4 and phosphate buffer saline (PBS) [pH 6.8, 8.4] but highly soluble in ethanol and dimethyl sulfoxide. Fourier transform infrared spectroscopy (FTIR) analysis of pure curcumin revealed the presence of different functional groups. In an attempt to optimize the best suited nanoparticles, three different formulations were prepared with increasing concentration of curcumin. Curcumin (CRN) @ 500 mg kg-1 was found to be the best formulation with particle size of 844 nm, zeta potential of -32.7 mV, encapsulation efficiency of 70.9%, and loading efficiency of 9.2%. Further, it was characterized by FTIR, X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) analysis. In vitro drug release studies showed the initial burst release followed by slow and steady release up to 100 h. Finally, pure CRN anticancer activity is compared with chitosan nanoparticles (CNPs) loaded with CRN. Percentage of cells viable decreased with increase in the concentration of both CRN and CRN-CNPs-1. CRN loaded in CNPs was founmore effective than pure CRN. Thus, the study revealed the successful encapsulation of curcumin and increasing therapeutic in in the treatment of breast cancers.
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Aggarwal, S., Verma, S.S., Aggarwal, S. and Gupta, S.C. 2021. Drug repurposing for breast cancer therapy: Old weapon for new battle. Seminars in Cancer Biology, 68: 8–20. Anjum, F., Razvi, N. and Masood, M.A. 2017. Breast cancer therapy: A mini review. MOJ Drug Design Development & Therapy, 1(2): 35-38.
Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., Linder, T., Wawrosch, C., Uhrin, P., Temml, V., Wang, L., Schwaiger, S., Heiss, E.H., Rollinger, J.M., Schuster, D., Breuss, J.M.,
Screening anticancer activity of curcumin loaded chitosan nanoparticles 133
Bochkov, V., Mihovilovic, M.D., Kopp, B., Bauer, R., Dirsch, V.M. and Stuppner, H. 2015. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances, 33(8): 1582–1614.
Baka, E., Comer, J.E. and Takács-Novák, K. 2008. Study of equilibrium solubility measurement by saturation shake-flask method using hydrochlorothiazide as model compound. Journal of Pharmaceutical and Biomedical Analysis, 46(2): 335-341.
Bayet-Robert, M., Kwiatkowski, F., Leheurteur, M., Gachon, F., Planchat, E., Abrial, C., Mouret Reynier, M.A., Durando, X., Barthomeuf, C. and Chollet, P. 2010. Phase I dose escalation trial of docetaxel plus curcumin in patients with advanced and metastatic breast cancer. Cancer Biology and Therapy, 9: 8-14.
Choi, Y.H., Han, D.H., Kim, S.W., Kim, M.J., Sung, H.H., Jeon, H.G., Jeong, B.C., Seo, S.I., Jeon, S.S., Lee, H.M. and Choi, H.Y. 2019. A randomized, double-blind, placebo-controlled trial to evaluate the role of curcumin in prostate cancer patients with intermittent androgen deprivation. Prostate, 79(6): 614-621.
Chuah, L.H., Billa, N., Roberts, C.J., Burley, J.C. and Manickam, S. 2013. Curcumin-containing chitosan nanoparticles as a potential mucoadhesive delivery system to the colon. Pharmaceutical Development and Technology, 18(3): 591-599.
Cruz-Correa, M., Hylind, L.M., Marrero, J.H., Zahurak, M.L., Murray-Stewart, T., Casero, R.A., Montgomery, E.A., Iacobuzio-Donahue, C., Brosens, L.A., Offerhaus, G.J., Umar, A., Rodriguez, L.M. and Giardiello, F.M. 2018. Efficacy and safety of curcumin in treatment of intestinal adenomas in patients with familial adenomatous polyposis. Gastroenterology, 155(3): 668-673.
Deman, J.M., Deman, L. and Blackman, B. 1983. Melting-point determination of fat products. Journal of the American Oil Chemists’ Society, 60(1): 91-94.
Fernandes, Q.M., Melo, K.R.T., Sabry, D.A., Sassaki, G.L. and Rocha, H.A.O. 2015. Does the use of chitosan contribute to oxalate kidney stone formation? Marine Drugs, 13(1): 141-158. Forato, L.A., Bernardes-Filho, R. and Colnago, L.A. 1998. Protein structure in KBr pellets by infrared spectroscopy. Analytical Biochemistry, 259(1): 136-141.
Gupta, S.C., Patchva, S., Koh, W. and Aggarwal, B.B. 2012. Discovery of curcumin, a component of golden spice, and its miraculous biological activities. Clinical and Experimental Pharmacology & Physiology, 39(3): 283-299.
Howells, L.M., Iwuji, C.O.O., Irving, G.R.B., Barber, S., Walter, H., Sidat, Z., Griffin-Teall, N., Singh, R., Foreman, N., Patel, S.R., Morgan, B., Steward, W.P., Gescher, A., Thomas, A.L. and Brown, K. 2019. Curcumin combined with FOLFOX chemotherapy is safe and tolerable in patients with metastatic colorectal cancer in a randomized phase IIa trial. The Journal of Nutrition, 149(7): 1133-1139.
Imad Uddin, M.D. and Veeresh, B. 2020. Systematic review on screening the role of chemosensitizer or synergistic drug and doxorubicin as dual drug loaded nanoparticle in overcoming multidrug resistant breast cancer. Annals of Phytomedicine, 9(2): 113-124.
Imad Uddin, M.D., Srikar, P.V.R., Preethi, Y.K., Rachana, C. and Deepika, R. 2020. Synthesis and characterization of chitosan nanoparticles loaded with 6-gingerol isolated from Zingiber officinale Rosc. Annals of Phytomedicine, 9(2): 164-171.
Jaiswal, S. and Mishra, P. 2018. Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with improved stability and selective toxicity to bacteria over mammalian cells. Medical Microbiology and Immunology, 207(1): 39-53.
Khan, M.A., Zafaryab, M.D., Mehdi, S.H., Ahmad, I. and Rizvi, M.M.A. 2016. Characterization and anti-proliferative activity of curcumin loaded chitosan nanoparticles in cervical cancer. International Journal of Biological Macromolecules, 93: 242-253.
Kunnumakkara, A.B., Bordoloi, D., Padmavathi, G., Monisha, J., Roy, N.K., Prasad, S. and Aggarwal, B.B. 2017. Curcumin, the golden nutraceutical: Multitargeting for multiple chronic diseases. British Journal of Pharmacology, 174: 1325-1348.
M.D. Imad Uddin and B. Veeresh
Mohammadi, K., Thompson, K.H., Patrick, B.O., Storr, T., Martins, C., Polishchuk, E., Yuen, V.G., McNeill, J.H. and Orvig, C. 2005. Synthesis and characterization of dual function vanadyl, gallium and indium curcumin complexes for medicinal applications. Journal of Inorganic Biochemistry. 99: 2217-2225.
Moudi, M., Go, R., Yien, C.Y.S. and Nazre, M. 2013. Vinca alkaloids. International Journal of Preventive Medicine, 4(11): 1231-1235.
Mohammed, M.A., Syeda, J., Wasan, K.M. and Wasan, E.K. 2017. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics, 9(4): 53. [https://doi.org/10.3390/pharmaceutics9040053].
Othman, N., Masarudin, M.J., Kuen, C.Y., Dasuan, N.A., Abdullah, L.C., Jamil, M. and Ain, S.N. 2018. Synthesis and optimization of chitosan nanoparticles loaded with l-ascorbic acid and thymoquinone. Nanomaterials, 8(11): 920. [doi: 10.3390/nano8110920].
Patel, V.R. and Agrawal, Y.K. 2011. Nanosuspension: An approach to enhance solubility of drugs. Journal of Advanced Pharmaceutical Technology & Research, 2(2): 81-87.
Savale, S.K. 2017. Curcumin as a model drug: conformation, solubility estimation, morphological, in vitro and in vivo biodistribution study. Journal of Pharma SciTech, 7: 31-35. Singh, A. and Avupati, V.R. 2017. Development and validation of UV-spectrophotometric method for the estimation of curcumin in standardized polyherbal formulations. Journal of Young Pharmacists, 9(4): 491-495
Sun, M., Gao, Y., Guo, C., Cao, F., Song, Z., Xi, Y., Yu, A., Li, A. and Zhai, G. 2010. Enhancement of transport of curcumin to brain in mice by poly(n-butylcyanoacrylate) nanoparticle. Nanoparticle Research, 12: 3111-3122.
Teli, M.D. and Sheikh, J. 2012. Extraction of chitosan from shrimp shells waste and application in antibacterial finishing of bamboo rayon. International Journal of Biological Macromolecules, 50(5): 1195-1200.
Tomaz, A.F., de Carvalho, S.M.S., Barbosa, R.C., Silva, S.M., Gutierrez, M.A.S., de Lima, A.G.B. and Fook, M.V.L. 2018. Ionically crosslinked chitosan membranes used as drug carriers for cancer therapy application. Materials, 11(10): 2051. [DOI: 10.3390/ma11102051].
Tomeh, M.A., Hadianamrei, R. and Zhao, X. 2019. A review of curcumin and its derivatives as anticancer agents. International Journal of Molecular Sciences, 20(5): 1033. [https://doi.org/10.3390/ijms20051033].
Udompornmongkol, P. and Chiang, B.H. 2015. Curcumin-loaded polymeric nanoparticles for enhanced anti-colorectal cancer applications. Journal of Biomaterials Applications, 30(5): 537-546.
Veeresham, C. 2012. Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Technology & Research, 3(4): 200-201
WHO. 2021. Cancer Fact Sheet 21, World Health Organization. Geneva, Switzerland. Accessed on 13-12-2021; [https://www.who.int/news-room/fact-sheets/detail/cancer]
Yallapu, M.M., Khan, S., Maher, D.M., Ebeling, M.C., Sundram, V., Chauhan, N., Ganju, A., Balakrishna, S., Gupta, B.K., Zafar, N. and Jaggi, M., 2014. Anti-cancer activity of curcumin loaded nanoparticles in prostate cancer. Biomaterials, 35(30): 8635-8648.
Yao, Y., Zhou, Y., Liu, L., Xu, Y., Chen, Q., Wang, Y., Wu, S., Deng, Y., Zhang, J. and Shao, A. 2020. Nanoparticle-based drug delivery in cancer therapy and its role in overcoming drug resistance.Frontiers in Molecular Biosciences, 7: 193. [https://doi.org/10.3389/fmolb.2020. 00193].
Yu, M., Yuan, W., Li, D., Schwendeman, A. and Schwendeman, S.P. 2019. Predicting drug release kinetics from nanocarriers inside dialysis bags. Journal of Controlled Release, 315: 23-30. Zhang, Y., Yang, C., Wang, W., Liu, J., Liu, Q., Huang, F., Chu, L., Gao, H., Li, C., Kong, D. and
Liu, Q. 2016. Co-delivery of doxorubicin and curcumin by pH-sensitive prodrug nanoparticle for combination therapy of cancer. Scientific Reports, 6(1): 1-12.
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