Green synthesis and characterization of Zinc oxide nanoparticle with polyspice extractantioxidant and antibacterial potential against oral pathogens
DOI:
https://doi.org/10.48165/aabr.2025.2.2.03Keywords:
oral infection, red complex pathogen, Fusobacterium, Zinc nanoparticle, AntibacterialAbstract
Because it is environmentally beneficial, the synthesis of metal oxide nanoparticles utilizing medicinal plants is growing quickly. The polyspice extract made by soxhlet extraction with ethylacetate is used in this research to create zinc oxide nanoparticles. GCMS and qualitative phytochimcal analysis were carried out. ZnONP and extract antioxidant activities were measured and compared to a standard. Using the disc diffusion approach, an antibacterial experiment was conducted against oral pathogens. Polyspice was found to include alkaloids, flavonoids, and phenol. Ten distinct secondary compounds were detected using GCMS. UV-Vis Spectroscopy analysis was used to describe the synthesized NPs, with a peak detected at 396-398 nm. SEM with EDX analysis verified the NPs’ spherical form 30-98 nm and the primary constituents are oxygen and zinc. At 50–100 µg levels, ZnO NPs inhibition assay on DPPH, metal chelation, and hydroxyl radical activity demonstrates good inhibition against free radicals. The ZnONPs that were biosynthesized showed higher antibacterial effectiveness than the standard at least 25µg, which is equivalent to standard chlorohexidine. The pattern of the nanoparticles’ antibacterial activity against the chosen bacteria was P. gingivalis, S. mutans, and Fusobacterium sp. According to the study’s findings, synthesizing zinc oxide nanoparticles with a spice extract is an economical and environmentally responsible way to produce green ZnO nanoparticles that have strong anti-oxidant and anti-microbial properties against oral infections.
References
American Society for Testing and Materials. (1999). Powder diffraction files. Joint Committee on Powder Diffraction Standards, Swarthmore, PA, 3.
Anees Ahmed, M., Srinivas, R., & Pramod, G. (2015). Studies on antimicrobial activity of spices and effect of temperature and pH on its antimicrobial properties. Journal of Pharmacy and Biological Sciences, 10(1), 99–110.
Chan, C. L., Gan, R. Y., Shah, N. P., & Corke, H. (2018). Polyphenols from selected dietary spices and medicinal herbs differentially affect common food-borne pathogenic bacteria and lactic acid bacteria. Food Control, 92, 437–443. DOI: https://doi.org/10.1016/j.foodcont.2018.05.032
Curtis, M. A., Aduse-Opoku, J., & Rangarajan, M. (2002). Attenuation of the virulence of Porphyromonas gingivalis by using a specific synthetic Kgp protease inhibitor. Infection and Immunity, 70, 6968–6975. DOI: https://doi.org/10.1128/IAI.70.12.6968-6975.2002
Datta, A., Patra, C., Bharadwaj, H., Kaur, S., & Dimri, N. (2017). Green synthesis of zinc oxide nanoparticles using Parthenium hysterophorus leaf extract and evaluation of their antibacterial properties. Journal of Biotechnology and Biomaterials, 7(3), 271–276. DOI: https://doi.org/10.4172/2155-952X.1000271
Dobrucka, R., Dlugaszewska, J., & Kaczmarek, M. (2018). Cytotoxic and antimicrobial effects of biosynthesized ZnO nanoparticles using Chelidonium majus extract. Biomedical Microdevices, 20(1), 5–18. DOI: https://doi.org/10.1007/s10544-017-0233-9
Fouda, A., Saad, E. L., Salem, S. S., & Shaheen, T. I. (2018). In-vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized zinc oxide nanoparticles for medical textile applications. Microbial Pathogenesis, 125, 252–261. DOI: https://doi.org/10.1016/j.micpath.2018.09.030
Gottardi, D., Bukvicki, D., Prasad, S., & Tyagi, A. K. (2016). Beneficial effects of spices in food preservation and safety. Frontiers in Microbiology, 7, 1394. DOI: https://doi.org/10.3389/fmicb.2016.01394
Gulcin, I., & Alwasel, S. H. (2023). DPPH radical scavenging assay. Processes, 11(8), 2248. DOI: https://doi.org/10.3390/pr11082248
Hajishengallis, G., Liang, S., & Payne, M. A. (2011). Low-abundance biofilm species orchestrates inflammatory periodontal disease through the commensal microbiota and complement. Cell Host & Microbe, 10, 497–506. DOI: https://doi.org/10.1016/j.chom.2011.10.006
Hossain, M. B., Brunton, N. P., Barry-Ryan, C., Martin-Diana, A. B., & Wilkinson, M. (2011). Antioxidant activity of spice extracts and phenolics in comparison to synthetic antioxidants. Rasayan Journal of Chemistry, 4, 751–756.
Indu, M. M., Hatha, A. A. M., Abirosh, C., Harsha, U., & Vivekanandan, G. (2006). Antimicrobial activity of some of the South Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Brazilian Journal of Microbiology, 37, 153–158. DOI: https://doi.org/10.1590/S1517-83822006000200011
Irshad, S., Ashfaq, A., Muazzam, A., & Yasmeen, A. (2017). Antimicrobial and anti-prostate cancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.) used in typical Pakistani cuisine. Pakistan Journal of Zoology, 49, 1665–1669. DOI: https://doi.org/10.17582/journal.pjz/2017.49.5.1665.1669
Ji, S., Choi, Y. S., & Choi, Y. (2015). Bacterial invasion and persistence: Critical events in the pathogenesis of periodontitis? Journal of Periodontal Research, 50, 570–585. DOI: https://doi.org/10.1111/jre.12248
Kim, I. S., Yang, M. R., Lee, O. H., & Kang, S. N. (2011). Antioxidant activities of hot water extracts from various spices. International Journal of Molecular Sciences, 12, 4120–4131. DOI: https://doi.org/10.3390/ijms12064120
Kubo, I., Fujita, K., Kubo, A., Nihei, K., & Ogura, T. (2004). Antibacterial activity of coriander volatile compounds against Salmonella choleraesuis. Journal of Agricultural and Food Chemistry, 52, 3329–3332. DOI: https://doi.org/10.1021/jf0354186
Linden, G. J., Lyons, A., & Scannapieco, F. A. (2013). Periodontal systemic associations: Review of the evidence. Journal of Periodontology, 84, S8–S19. DOI: https://doi.org/10.1902/jop.2013.1340010
Liu, P. F., Haake, S. K., Gallo, R. L., & Huang, C. M. (2009). A novel vaccine targeting Fusobacterium nucleatum against abscesses and halitosis. Vaccine, 27(10), 1589–1595. DOI: https://doi.org/10.1016/j.vaccine.2008.12.058
Maharjan, R., Thapa, S., & Acharya, A. (2019). Evaluation of antimicrobial activity and synergistic effect of spices against a few selected pathogens. Tribhuvan University Journal of Microbiology, 6(1), 10–18. DOI: https://doi.org/10.3126/tujm.v6i0.26573
Mofid, H., Sadjadi, M. S., Sadr, M. H., Banaei, A., & Farhadyar, N. (2020). Green synthesis of zinc oxide nanoparticles using Aloe vera plant for investigation of antibacterial properties. Advances in Nanochemistry, 2(1), 32–35.
Naseer, M., Aslam, U., Khalid, B., & Chen, B. (2020). Green route to synthesize zinc oxide nanoparticles using leaf extracts of Cassia fistula and Melia azedarach and their antibacterial potential. Scientific Reports, 10, 9055. DOI: https://doi.org/10.1038/s41598-020-65949-3
Park, M., Bae, J., & Lee, D. (2008). Antibacterial activity of gingerol isolated from ginger rhizome against periodontal bacteria. Phytotherapy Research, 22, 1446–1449. DOI: https://doi.org/10.1002/ptr.2473
Porter, S. R. (2011). Diet and halitosis. Current Opinion in Clinical Nutrition and Metabolic Care, 14(5), 463–468. DOI: https://doi.org/10.1097/MCO.0b013e328348c054
Raghupathi, K. R., Koodali, R. T., & Manna, A. C. (2011). Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir, 27, 4020–4028. DOI: https://doi.org/10.1021/la104825u
Rajendran, S. P., & Sengodan, K. (2017). Synthesis and characterization of zinc oxide and iron oxide nanoparticles using Sesbania grandiflora leaf extract as reducing agent. Journal of Nanoscience, 2017, 1–7. DOI: https://doi.org/10.1155/2017/8348507
Savunthari, K. V., Arunagiri, D., Shanmugam, S., Ganesan, S., Arasu, M. V., & Al-Dhabi, N. A. (2021). Green synthesis of lignin nanorods/g-C₃N₄ nanocomposite materials for efficient photocatalytic degradation of triclosan in environmental water. Chemosphere, 272, 129801. DOI: https://doi.org/10.1016/j.chemosphere.2021.129801
Soren, S., Kumar, S., Mishra, S., Jena, P. K., Verma, S. K., & Parhi, P. (2018). Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method. Microbial Pathogenesis, 119, 145–151. DOI: https://doi.org/10.1016/j.micpath.2018.03.048
Tachakittirungrod, S., Okonogi, S., & Chowwanapoonpohn, S. (2007). Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chemistry, 103, 381–388. DOI: https://doi.org/10.1016/j.foodchem.2006.07.034
Vergara-Llanos, D., Koning, T., Pavicic, M. F., Bello-Toledo, H., Díaz-Gómez, A., Jaramillo, A., Melendrez-Castro, M., Ehrenfeld, P., & Sánchez-Sanhueza, G. (2021). Antibacterial and cytotoxic evaluation of copper and zinc oxide nanoparticles as a potential disinfectant material of connections in implant provisional abutments: An in-vitro study. Archives of Oral Biology, 122, 105031. DOI: https://doi.org/10.1016/j.archoralbio.2020.105031
Zhao, J. G., Yang, K. C., Yang, L., Chen, Y. P., Sun, R., Guo, J. X., & Li, D. Q. (2020). A mixed-ligand Zn(II)-based coordination polymer for selective detection of 2,4,6-trinitrophenol (TNP) and treatment effect on periodontal diseases via inhibition effect on P. gingivalis growth. Journal of Oleo Science, 69, 115–122. DOI: https://doi.org/10.5650/jos.ess19204
