Study of Carbapenemase Producing Non-Glucose Fermenters in Tertiary Care Hospital

Indian Journal of Applied Basic Medical Sciences
Year: 2020, Volume: 22(A), Issue. (1)
First page: (150) Last page: (157)
Online ISSN: 2249-7935
Print ISSN: 0975-8917
doi:10.48165/ijabms.2020.22114

Study of Carbapenemase Producing Non-Glucose Fermenters in Tertiary Care  Hospital 
Sravanthi Reddy Pagala 1, Mina Kadam2, Sanjay Rathod3
1Ph.D scholar, Gujarat University,  2 Professor & Head, 3 Associate Prof AMC MET Medical college, LG Hospital, Gujarat University
Corresponding author email id:

Online Published on 02-Jan-2020

ABSTRACT

Carbapenems have been used as the last resort antimicrobials in the treatment of serious  infections caused by gram negative bacteria. The rate of carbapenem resistance in non-glucose  fermenting gram negative bacilli has been gradually increasing worldwide over last 10 years.  These organisms can also cause life threating infections which are difficult to treat. Aim: This study was conducted to identify phenotypically for the presence of Carbapenemase  production in glucose non-fermenting bacilli. Materials and methods: Among 200 Imipenem resistant isolates, 62 imipenem resistant  samples showed glucose non-fermenting bacilli. All the isolates were tested for anti-microbial  susceptibility (HI-Media Mumbai) by Kirby-Bauer disk diffusion method on Muller-Hinton agar.  Carbapenemase production was detected by Modified-Hodge test, Combined Disc test, and  AmpC Disc test phenotypically. Results. Out of 62 imipenem resistant isolates of glucose non-fermenting bacilli, 49 isolates  were Pseudomonas spp. and 13 isolates were Acinetobacter spp.. Out of 62 , 13 were MHT  positive, 36 were CDT positive and 14 were AmpC positive, 16 were Multiple enzyme producers,  17 were Non-Enzyme producers. Conclusion: Carbapenem resistance rate was more in Acinetobacter spp. than Pseudomas spp.. Our study has implicated the severity of carbapenem resistant non-fermenters, which are  effecting the infection control activities among hospital setup. 

Keywords

MHT=Modified Hodge test, CDT=Combined Disc test, MBL=Metallo betalactamase